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KMID : 0613820060160030459
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Journal of Life Science
2006 Volume.16 No. 3 p.459 ~ p.463
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Induction of Shoot Primordium in Culture of Garlic (Allium sativum L.)
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Choi Joo-Soo
Lee Bok-Kyu
Huh Man-Kyu
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Abstract
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Cultivated garlic, Allium sativum L. is economically important for leaves and bulbs, which historically were used in Korea for spices and condiments of Korean food as well as medicine crops. This experiment was carried out to investigate the effect of development and differentiation on culture of A. sativum (cv: white 6) by explant position, hormone composition and sucrose concentration in culture media. Culture method was investigated to induce shoot primordium. Culture efficiency was better with lower tissue of foliage leaf in explant position and on the medium with NAA 0.02 + BAP 1.0 ㎎/ℓ in hormone composition than any other. Precocious shoot and callus were induced from shoot apex. Shoot was efficiently differentiated on 4,000 ㎎/ℓ sucrose with increasing concentration of BAP. Shoot primordium was also induced with liquid rotary culture by histological observation. Rhizoid was induced from callus tissue cluster on medium with NAA 0.02 + BAP 2.0 ㎎/ℓ.
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KEYWORD
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Garlic (Allium sativum L.), liquid rotary culture, shoot primordium
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